Kirby Robbins scored in the 83rd minute Thursday night, lifting host and fifth-ranked Messiah to a 1-0 win against No. 9 Johns Hopkins in Grantham, Pennsylvania.
The loss was the first this season for the Blue Jays (11-1), who had outscored their opponents 27-2 coming into the contest and ranked third nationally among Division III teams in goals allowed per game.
Hopkins outshot Messiah 10-9, and JHU goalkeeper Bryan See made three saves.
The goal was Robbins' ninth of the season for the Falcons (11-1).
This was the first top-10 matchup for Johns Hopkins since Sept. 8, 2010, when sixth-ranked JHU defeated No. 10 York 2-1 in double overtime.
The Blue Jays return to action on Saturday at Swarthmore.