Department of Biology Seminar Series: Bernd Bukau

Description

Bernd Bukau, a research group leader at the Center of Molecular Biology at Heidelberg University and the German Cancer Research Center, will present a talk titled "Co-Translational Folding and Assembly of Newly Synthesized Proteins" for the Department of Biology.

This is a hybrid event; to attend virtually, use the Zoom link.

Abstract:

Many of the critical steps of enzymatic processing, membrane targeting, native folding and even assembly of newly synthesized proteins occur co-translationally, implying that protein maturation and decoding of genetic information by the ribosome are coupled processes. We are mainly using ribosome profiling technology in bacteria, yeast and human cells to dissect genome-wide protein maturation steps at nearcodon resolution and in dependence of the length of the nascent polypeptide chains. This approach allowed us to dissect the flux of the cellular nascent chain proteome through the system of co-translationally engaged chaperones and to demonstrate that many oligomeric protein complexes assembly cotranslationally. Recently, we found evidence for the coordination of translation speed and assembly.